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A hindbrain segmental scaffold specifying neuronal location in the adult goldfish, Carassius auratus

J Comp Neurol 522: 2446-64. doi: 10.1002/cne.23544.

Authors/Editors: Gilland E
Straka H
Wong TW
Baker R
Zottoli SJ
Publication Date: 2014
Type of Publication: Journal Articles 2001 - 2017

Abstract

The vertebrate hindbrain develops as a series of well-defined neuroepithelial segments or rhombomeres. While rhombomeres are visible in all vertebrate embryos, there is not generally any visible segmental anatomy in the brains of adults. Teleost fish are exceptional in retaining a rhombomeric pattern of reticulospinal neurons through embryonic, larval and adult periods. We use this feature to more precisely map the segmental imprint in the reticular and motor basal hindbrain of adult goldfish. Analysis of serial sections cut in three planes and computer reconstructions of retrogradely labeled reticulospinal neurons yielded a segmental framework compatible with previous reports and more amenable to correlation with surrounding neuronal features. Cranial nerve motoneurons and octavo-lateral efferent neurons were aligned to the reticulospinal scaffold by mapping neurons immunopositive for choline acetyltransferase or retrogradely labeled from cranial nerve roots. The mapping corresponded well with the known ontogeny of these neurons and helps confirm the segmental territories defined by reticulospinal anatomy. Since both the reticulospinal and motoneuronal segmental patterns persist in the hindbrain of adult goldfish, we hypothesize that a permanent "hindbrain framework" may be a general property that is retained in adult vertebrates. The establishment of a relationship between individual segments and neuronal phenotypes provides a convenient method for future studies that combine form, physiology and function in adult vertebrates. J. Comp. Neurol., 2014. © 2014 Wiley Periodicals, Inc.

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